Reporter

Part:BBa_I715028:Design

Designed by: Jessica Treece   Group: iGEM07_Davidson_Missouri_W   (2007-07-17)


pLac-RBS-Kan-RBS-RFP-RBS-Tet


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1944
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2090
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2116
    Illegal NgoMIV site found at 2484
    Illegal NgoMIV site found at 2644
    Illegal AgeI site found at 1623
    Illegal AgeI site found at 1735
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A strong RBS was used (B0034) in front of each coding sequence.


Source

Composed of existing registry parts.

References